Shawn Mikula on Brain Preservation Protocols and Extensions

Here's an interview with Shawn Mikula  a neuroscientist on his recent paper in Nature Methods describing a new protocol for preserving a mouse brain such that, in principle, the entire neuronal connectome can be scanned (actually doing it using current technology would take years).

The work is exciting because it shows that in as little as 3 years, a similar protocol for humans could be devised. That'd pave the way for mind uploading and other advances in neuroscience.

Here we describe a preparation, BROPA (brain-wide reduced-osmium staining with pyrogallol-mediated amplification), that results in the preservation and staining of ultrastructural details throughout the brain at a resolution necessary for tracing neuronal processes and identifying synaptic contacts between them. Using serial block-face electron microscopy (SBEM), we tested human annotator ability to follow neural ‘wires’ reliably and over long distances as well as the ability to detect synaptic contacts. Our results suggest that the BROPA method can produce a preparation suitable for the reconstruction of neural circuits spanning an entire mouse brain

Comparison OF BROPA to the similar but older ROTO

http://blog.brainpreservation.org/2015/04/27/shawn-mikula-on-brain-preservation-protocols/

How to sign up for Alcor cryo

A few months ago, I signed up for Alcor's brain-only cryopreservation. The entire process took me 11 weeks from the day I started till the day I received my medical bracelet (the thing that’ll let paramedics know that your dead body should be handled by Alcor). I paid them $90 for the application fee. From now on, every year I’ll pay $530 for Alcor membership fees, and also pay $275 for my separately purchased life insurance.

This article is intended for those who already think cryopreservation is a good idea but are putting it off since they don't know exactly what needs to be done. As you'll see from the cryopreservation agreement (page 10), the procedure is still experimental so there's a good chance that you won't be revived even if you are "frozen" the moment you die.

I chose to go the neurocryopreservation route since it seems to offer better chances for a high quality preservation, and also since it will be slightly easier for your remains to be evacuated from the storage facility in case of an emergency.

The process

Fill out the Alcor membership application available at the Alcor Membership Information and Enrollment Instructions Page. You'll have to pay the application fee of $90 to continue the process by filling out your credit card details on the last page. If you want to pay in a different manner, like I did, just call them directly at 480-905-1906 and someone will take your money.

Sign the cryo contract and get it cosigned

Next Alcor'll physically mail you the cryopreservation contracts to read and sign. Since the document runs to 60 pages, you'll need about 2 hours to read through everything. Once you're done, you'll need 3 witnesses to sign the contract. If you live in California, you will NOT need a notary to sign it.

Apply for life insurance

In order to pay for my cryopreservation, I chose to use a life insurance policy. You can use the life insurance policy offered by your employer, but only if the policy is portable i.e. you can change the legal owner and the legal beneficiary of the policy to Alcor.

I simply went to the GEICO Website and looked around for a life insurance policy that didn't require me to submit to a physical exam first. I couldn't complete the application online but a representative called me and got me insured for $22.50 per month within 24 hours.

I've heard from others who are signed up for cryo that Rudi Hoffman, a financial planner, gets good life insurance deals and will also help with the paperwork.

Change the beneficiary and owner of the insurance policy to Alcor

Once you have your life insurance policy, you'll need to change the beneficiary and owner to Alcor so that they receive the benefits in the event of your death. You'll need to

1. Request the required forms from your insurance company
2. Fill out, return, and confirm receipt of the change of owner form first
3. Then fill out and return the change of beneficiary form

This was the most frustrating part of the process since I had to call the insurance company weekly for more than a month to check on the status of my forms. Note that the Alcor folks will help you fill out your change of ownership and beneficiary forms. Just ask.

Receive your Alcor medallion

Once you've confirmed that your policy has been changed, let Alcor know and they'll send you a membership packet containing a necklace and a bracelet for you to wear. In case you're involved in a fatal accident, the necklace should inform paramedics of how to treat your body until Alcor arrives.

If you'll frequently be travelling outside the U.S., ask Alcor to send you a necklace that has a phone number with the American country code prefix on it.

Alcor necklace (front)

Alcor necklace (reverse)

Extras

The preceding is all you need to be fully signed up. For bonus points, you can also do the following:

1. Get your family members to sign affidavits promising not to block Alcor’s preservation activities after you're dead.
2. Create a trust fund for when you’re revived.

Picking the Right Fields

One of my goals is to see the world in a hundred years from now, that is 2115. Sadly, even if I reached the maximum observed human lifespan of 122 years, I’d still only make it another 90 years.

Seeing 2115 requires three things to occur: live long enough for the right technology to be developed, make humans not have to die anymore, and avoid global catastrophic risks. Here are my very rough estimates for the minimum amount of money that needs to be spent over the next 10 years to solve each problem

• Live long: $0.5 million

• No death: $1MM

• No catastrophes: greater than $10 billion

Live long enough for the right technology to be developed

I need about $50,000 annually to live a good life. This involves purchasing creature comforts and signing up for cryonics. 10 years of this gives $0.5 million. Sadly, cryonics is not yet guaranteed to work, otherwise I could just put myself to sleep right now for much less money.

Make humans not have to die anymore

I see two paths to this

1. Suspended animation: This involves preserving the body (or at least the brain) before it is information theoretically dead. Through private conversations with researchers in chemo- and cryo-preservation I learnt that it’ll take roughly $1 million and 3 years to develop procedures to preserve a human brain. This assumes, of course, that the vast majority of what makes us human lies in our brains.
2. SENS research program: The SENS research foundation funds a research program at many different universities to cure aging by reversing the cellular damage that accumulates over our lifetimes. Their founder estimates that it’ll take $200 million to $1 billion dollars over 10 years to make the fastest progress on solving the problem of death.

Avoid global catastrophic risks

The few risks I looked into all would cost more than $10 billion over the next 10 years to avert. For instance, the 2015 U.S. budget has $1.6 billion for nuclear non-proliferation and at least $1.2 billion for biodefense. Surprisingly, artificial intelligence risk had no estimate I could find.

Next Actions

I’ve begun working on extending my healthy life span by increasing exercise, eating better, avoiding smoking, and driving more carefully. 

I’m also volunteering with the Brain Preservation Foundation, which funds research into cryonics and other means of brain preservation. Hopefully we’ll spur even better preservation protocols to be developed so that within a decade far fewer people will have to die any more.

FAI research seems potentially high leverage as well despite, or maybe because of, its unknown costs.There are very few people working on the related problems now (I couldn’t even find a precise definition of control problem), and yet it requires no specialized equipment, just a brain and an Internet connection. This suggests that trying to solve FAI-related problems could be unusually useful.

I suspect that a lot of people will be opposed or indifferent to the idea of life extension until such a point that the technology is incontrovertibly feasible. And by that point, there'd no longer be any need for public approval of life extension.

We in the life extension community might increase the effectiveness of our marketing by explicitly conflating life extension, healthspan extension, and the promise of experience a much, much better future. In other words, eternal life in heaven. In too many conversations once I raise the possibility of life extension, the next objections are either, Why would you want to live forever as a frail old person? or How will we feed all those humans on earth if no one dies?

Perhaps I'd pique more people's interests if I led with, "the next 100 years are gonna be so great: we'll cure all diseases and have vacations on Mars and no one will have to work any more. That's why I'm working on biotechnologies that will reverse the aging in people today; so that we can all enjoy the wonderful things that are yet to come."

For the past few months I've been thinking about ways to get artificial intelligence (AI) solve the hard problems in biology since I'm having so much trouble solving them myself. This article from the Future of Life Institute, an AI think-tank points out that others have started out with similar intentions but have come to realize that they need to take particular care not to unleash a disaster in the process:

Human reasoning is based on an understanding derived from a combination of personal experience and collective knowledge derived over generations, explains MIRI researcher Nate Soares, who trained in computer science in college. For example, you don’t have to tell managers not to risk their employees’ lives or strip mine the planet to make more paper clips. But AI paper-clip makers are vulnerable to making such mistakes because they do not share our wealth of knowledge. Even if they did, there’s no guarantee that human-engineered intelligent systems would process that knowledge the same way we would.

I'd always thought that no one would be stupid enough to attach a superintelligent computer to actuators that could seriously affect the world but I've come to see that that is naive. The moment an AI is shown to be successful in a small field, it will almost instantly be given greater and greater responsibilities so that its owners can reap maximum rewards. 

Here's a 40 minute video from the Alcor Conference in 2006 giving a nice, straight-to-the-point overview of the process (and current state-of-the-art) of cryopreservation. It also explains the reasons why cryonics is thought to be a reasonable means of reaching the far future.

Alcor Conference 2006 - Brian Wowk, Ph.D. - The Cryobiological Basis of Cryonics (42 min) from Alcor Life Extension Foundation on Vimeo.

The Cat in the Hat On Aging

I found this Seussian poem on the Internet but can't attribute it. Oh well, Happy 2015!

I cannot see

I cannot pee

I cannot chew

I cannot screw

Oh my god, what can I do?

My memory shrinks

My hearing stinks

No sense of smell

I look like hell

My mood is bad - can you tell?

My body's drooping

Have trouble pooping

The Gold Years

have come at last

The Golden Years

can kiss my ass

I recently discovered the blog of a life extension activist, Maria Konovalenko, that has lots of cool media, plans, and links to others in the community. She is, for instance, affiliated with the (Russian) Science for Life Extension foundation, and she also cofounded Labcures, a site for crowdfunding individual scientific experiments.

Below is a series of posters that she commissioned to show the possible paths to immortality.

This open letter by the folks at the Brain Preservation Foundation was quite stirring, and pretty succinctly described why preserving dying people is beneficial,  and feasible with current technology.

As an aside, I learned from the letter that there are surgical procedures that stop all brain and circulatory activity in a patient and yet the patient can recover with their memories intact. This suggests that what makes us us can be preserved by a structural copy of the brain.

We choose brain preservation over natural decay because we accept the current scientific consensus that our unique conscious self is generated by processes within our physical brain. Further, we accept that all the memories, skills, and personality traits that make us unique are hardwired into the physical and molecular connections among our brain’s hundred billion neurons. Such a structural basis of memory and personality is demonstrated by the fact that surgical patients are often put into a state of Profound Hypothermia and Circulatory Arrest (PHCA) in which all patterned brain activity is halted for up to a full hour, yet these patients revive with memory and personality completely intact. The structural basis of memory and personality – the synaptic connectivity between neurons – can be preserved essentially perfectly by today’s chemical fixation and plastic embedding techniques. Extrapolating from current technologies for the nano‐imaging of plastic embedded brain tissue, we believe that one day science will have advanced sufficiently to allow complete retrieval of memories from such a preserved brain. 

I just ran into this 15-minute documentary that I think does a good job describing the motivation for pursuing anti-aging research. It is also wonderful to see how many established research institutions are working on the problem.